Immunocytochemical and ultrastructural studies of eosinophilic granular bodies in astrocytic tumors

Acta Neuropathol. 1992;83(4):408-14. doi: 10.1007/BF00713533.


Eosinophilic granular bodies (EGBs) are studied immunocytochemically and ultrastructurally in a case of low-grade and a case of high-grade astrocytoma. EGBs are recognized as brightly eosinophilic round bodies of variable size in hematoxylin and eosin-stained sections. Immunocytochemically some EGBs are positive for antibodies raised against alpha B-crystallin, ubiquitin and glial fibrillary acidic protein with the staining patterns for each being different from one another. Ultrastructurally EGBs consist of membrane-bound round body of various diameter ranging from 50 nm to 20 microns. Small EGBs contain electron-dense homogeneous material with occasional myelin figures, while electron-dense homogeneous material or loose granular profiles. Our studies demonstrate (1) ultrastructural variety of EGB; (2) and alpha B-crystallin epitope in EGB; and (3) the presence of EGB in high-grade as well as low-grade astrocytoma.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antibodies
  • Antibodies, Monoclonal
  • Astrocytoma / pathology*
  • Astrocytoma / ultrastructure
  • Brain Neoplasms / pathology*
  • Brain Neoplasms / ultrastructure
  • Child
  • Crystallins / analysis
  • Cytoplasmic Granules / ultrastructure*
  • Eosinophils / pathology*
  • Eosinophils / ultrastructure
  • Glial Fibrillary Acidic Protein / analysis
  • Humans
  • Immunohistochemistry
  • Intermediate Filaments / ultrastructure
  • Male
  • Microscopy, Electron


  • Antibodies
  • Antibodies, Monoclonal
  • Crystallins
  • Glial Fibrillary Acidic Protein