Identification and characterization of a putative cyclic nucleotide-gated channel, CNG-1, in C. elegans

Mol Cells. 2005 Feb 28;19(1):149-54.


Cyclic nucleotide-gated (CNG) channels encoded by the tax-4 and tax-2 genes are required for chemosensing and thermosensing in the nematode C. elegans. We identified a gene in the C. elegans genome, which we designated cng-1, that is highly homologous to tax-4. Partial CNG-1 protein tagged with green fluorescent protein was expressed in several sensory neurons of the amphid. We created a deletion mutant of cng-1, cng-1 (jh111), to investigate its in vivo function. The mutant worms had no detectable abnormalities in terms of their basic behavior or morphology. Whereas tax-4 and tax-2 mutants failed to respond to water-soluble or volatile chemical attractants, the cng-1 null mutant exhibited normal chemotaxis to such chemicals and a tax-4;cng-1 double mutant had a similar phenotype to tax-4 single mutants. Interestingly, cng-1 and tax-4 had a synergistic effect on brood size.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / physiology
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / isolation & purification*
  • Caenorhabditis elegans Proteins / physiology
  • Chemotaxis / genetics
  • Gene Deletion
  • Ion Channels / genetics
  • Ion Channels / isolation & purification*
  • Ion Channels / physiology
  • Molecular Sequence Data
  • Mutation
  • Neurons / metabolism
  • Odorants
  • Reproduction


  • CNG-1 protein, C elegans
  • Caenorhabditis elegans Proteins
  • Ion Channels
  • tax-2 protein, C elegans
  • tax-4 protein, C elegans