Targeted expression of Cre recombinase in macrophages and osteoclasts in transgenic mice

Genesis. 2005 Mar;41(3):138-45. doi: 10.1002/gene.20108.

Abstract

To develop specific conditional gene ablation in the hematopoietic myeloid-osteoclast lineage, transgenic mice expressing Cre recombinase under the control of the CD11b promotor were generated on the C57BL/6 background. The cellular specificity of Cre activity following recombination was quantified in the Z/EG reporter transgenic mice by FACS analysis with lineage-specific markers and EGFP coexpression. A high degree of recombination, as evidenced by EGFP-positive cells, was demonstrated in macrophages and granulocytes of bone marrow and spleen by the presence of double-positive cells CD11b/EGFP and Gr1/EGFP, respectively. Interestingly, the peritoneal macrophage population showed almost complete DNA recombination at large. Most important, mature osteoclast cells derived from the double transgenic bone marrow and spleen progenitors were EGFP-positive. Hence, these CD11b-Cre mice will provide a unique tool to unravel novel gene function and activities involved during osteoclast and macrophage differentiation and maturation processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD11b Antigen / biosynthesis
  • CD11b Antigen / genetics*
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • DNA, Recombinant
  • Flow Cytometry
  • Gene Expression Regulation
  • Green Fluorescent Proteins / biosynthesis
  • Integrases / biosynthesis*
  • Integrases / genetics*
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic / metabolism*
  • Models, Animal
  • Osteoclasts / metabolism*
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / biosynthesis
  • Stem Cells

Substances

  • CD11b Antigen
  • DNA, Recombinant
  • Recombinant Fusion Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Cre recombinase
  • Integrases