Efficacy of DNA vaccination against western equine encephalitis virus infection

Vaccine. 2005 Mar 18;23(17-18):2280-3. doi: 10.1016/j.vaccine.2005.01.032.

Abstract

The efficacy of a DNA vaccine against western equine encephalitis (WEE) infection in mice was evaluated. The 26S structural region was expressed, in vitro from an internal T7 promoter using a rabbit reticulysate transcription/translation system; and from a CMV promoter after transfection into Vero cell monolayers. The proteins synthesized were reactive with anti-WEE virus (WEEV) antibodies, both in western blot analysis and histochemical staining, respectively. When the DNA vaccine plasmid, pVHX-6, was administered intraepidermally to mice, followed by challenge in a lethal mouse model, the level of protection obtained ranged from 50 to 100% amongst three strains of WEEV. Preliminary results suggest the protective immunity provided by the DNA vaccine appears to be a cell-mediated immune response, as elevated cytotoxic T lymphocyte activity was detected against the E2 protein in a T-cell proliferation assay. The efficacy results suggest a DNA vaccine may be a promising approach against WEE infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / genetics
  • Chlorocebus aethiops
  • Encephalitis Virus, Western Equine / genetics
  • Encephalitis Virus, Western Equine / immunology*
  • Encephalomyelitis, Equine / immunology
  • Encephalomyelitis, Equine / prevention & control*
  • Genetic Vectors
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Plasmids / genetics
  • Rabbits
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccines, DNA / genetics
  • Vaccines, DNA / pharmacology
  • Vero Cells
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / immunology
  • Viral Vaccines / genetics
  • Viral Vaccines / pharmacology*

Substances

  • Antigens, Viral
  • Vaccines, DNA
  • Viral Structural Proteins
  • Viral Vaccines