In vitro effect of karathane LC (dinocap) on human lymphocytes

Mutagenesis. 2005 Mar;20(2):101-4. doi: 10.1093/mutage/gei013. Epub 2005 Mar 8.

Abstract

Karathane LC (active ingredient dinocap), a contact fungicide and a non-systemic acaricide was investigated for its ability to induce chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) in cultured human lymphocytes of peripheral blood. In addition to the cytogenetic analysis, the effect of karathane LC on the cell proliferation kinetics (CPK) by the replication index (RI) was studied. The mitotic index (MI) was also determined to detect the cytotoxic effect. Lymphocytes were treated with four different concentrations (5, 10, 15 and 20 microg/ml) of karathane LC for 24 and 48 h. Significant differences between exposed and non-exposed groups found in CAs, SCEs and MI demonstrate the mutagenic, clastogenic and also the cytotoxic effect of karathane LC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Proliferation / drug effects
  • Chromosome Aberrations / drug effects
  • Dinitrobenzenes / toxicity*
  • Female
  • Fungicides, Industrial / toxicity*
  • Humans
  • In Vitro Techniques
  • Insecticides / toxicity
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Male
  • Mitotic Index
  • Mutagenicity Tests
  • Mutagens / toxicity*
  • Sister Chromatid Exchange / drug effects

Substances

  • Dinitrobenzenes
  • Fungicides, Industrial
  • Insecticides
  • Mutagens
  • dinocap