Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin

K Kohri; Y Suzuki; K Yoshida; K Yamamoto; N Amasaki; T Yamate; T Umekawa; M Iguchi; H Sinohara; T Kurita

doi:10.1016/0006-291x(92)90669-c

Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin

Biochem Biophys Res Commun. 1992 Apr 30;184(2):859-64. doi: 10.1016/0006-291x(92)90669-c.

Authors

K Kohri¹, Y Suzuki, K Yoshida, K Yamamoto, N Amasaki, T Yamate, T Umekawa, M Iguchi, H Sinohara, T Kurita

Affiliation

¹ Department of Urology and Biochemistry, School of Medicine, Kinki University, Osaka, Japan.

PMID: 1575754
DOI: 10.1016/0006-291x(92)90669-c

Abstract

We have sequenced a cDNA of urinary stone protein. cDNA sequences show complete homology between urinary stone protein and human osteopontin (bone sialoprotein) (nucleotides 265-886 and 1183-1424). Osteopontin is a recently discovered bone matrix protein which has been implicated in mediating mineral formation within bone extracellular matrix. This result shows that osteopontin is presumably involved in stone formation as stone matrix.

Publication types

Comparative Study

MeSH terms

Base Sequence
Cloning, Molecular / methods
DNA / genetics*
Gene Library
Humans
Kidney / physiology
Molecular Sequence Data
Osteopontin
Phosphoproteins / genetics
Sequence Homology, Nucleic Acid
Sialoglycoproteins / genetics*

Substances

Phosphoproteins
SPP1 protein, human
Sialoglycoproteins
Osteopontin
DNA