Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism

I O Andreev; K V Spiridonova; V T Solovyan; V A Kunakh

doi:10.1016/j.cellbi.2004.11.002

Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism

Cell Biol Int. 2005 Jan;29(1):21-7. doi: 10.1016/j.cellbi.2004.11.002. Epub 2005 Jan 20.

Authors

I O Andreev¹, K V Spiridonova, V T Solovyan, V A Kunakh

Affiliation

¹ Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, Academy Zabolotnogo Street, 150, Kiev-143, 03143 Ukraine. i.o.andreev@imbg.org.ua

PMID: 15763495
DOI: 10.1016/j.cellbi.2004.11.002

Abstract

An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Ribosomal / genetics
Deoxyribonuclease BamHI / metabolism
Gene Rearrangement / genetics*
Genes, Plant*
Genes, rRNA*
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
RNA, Ribosomal / genetics
RNA, Ribosomal, 18S / genetics
RNA, Ribosomal, 5S / genetics
Rauwolfia / genetics*
Species Specificity
Tissue Culture Techniques

Substances

DNA, Ribosomal
RNA, Ribosomal
RNA, Ribosomal, 18S
RNA, Ribosomal, 5S
RNA, ribosomal, 25S
Deoxyribonuclease BamHI