Phylogenetic and biochemical characterisation of a recombinant laccase from Trametes versicolor

FEMS Microbiol Lett. 2005 Mar 15;244(2):235-41. doi: 10.1016/j.femsle.2005.01.054.

Abstract

Laccases are important enzymes for bioremediation and the best-characterised are from the fungus Trametes versicolor. Here, we describe the cloning and characterisation of a new variant of laccase from T. versicolor and its expression in Saccharomyces cerevisiae. We have performed a sequence-based analysis of Trametes laccases that leads to a proposal for a new nomenclature of this fungus laccases according to their phylogenetic relationships since their nomenclature based on IPs is ambiguous. We also describe the kinetic properties of the recombinant enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Basidiomycota / enzymology*
  • Basidiomycota / genetics
  • Biodegradation, Environmental
  • Laccase / biosynthesis
  • Laccase / chemistry
  • Laccase / classification*
  • Laccase / genetics
  • Molecular Sequence Data
  • Phylogeny
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / classification
  • Recombinant Proteins / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Recombinant Proteins
  • Laccase