Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway

J Am Chem Soc. 2005 Mar 23;127(11):3682-3. doi: 10.1021/ja042792t.


The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anthranilate Synthase / metabolism
  • Bacillus subtilis / enzymology
  • Bacillus subtilis / metabolism
  • Bacterial Proteins / metabolism
  • Fourier Analysis
  • Glutaminase / metabolism
  • Nitrogenous Group Transferases / metabolism
  • Pyridoxal Phosphate / biosynthesis*
  • Spectrometry, Mass, Electrospray Ionization


  • Bacterial Proteins
  • Pyridoxal Phosphate
  • Nitrogenous Group Transferases
  • Glutaminase
  • Anthranilate Synthase
  • anthranilate synthase, glutamine amidotransferase subunit