PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage

J Pathol. 2005 May;206(1):76-86. doi: 10.1002/path.1752.


PRDM1/Blimp-1 (in human and mouse, respectively) has a central role in determining and shaping the secretory arm of mature B-cell differentiation. In this study, a mouse monoclonal antibody that recognizes PRDM1 was used to detail its distribution in normal human lymphoid tissue and in lymphoid neoplasms that correspond to different stages of B-cell differentiation. PRDM1 was expressed in germinal centre blasts that co-express Pax5, CD19, CD20, and CD10, but not BCL6 or MTA-3. Pax5 was downregulated and full plasma cell morphology and phenotype were acquired by PRDM1+, nuclear cREL-, pre-plasma cells upon exit from the germinal centre. Activated extrafollicular B-cells (CD30+, Pax5+) were largely PRDM1-. PRDM1 was also absent in tissue histiocytes and the majority of resting T-cells and S-100+ antigen-presenting cells. PRDM1 and CD138 were expressed simultaneously in human lymphomas with plasma cell differentiation, but not in marginal zone lymphomas or chronic lymphocytic leukaemias. A minority of diffuse large B-cell lymphomas expressed PRDM1 and Hodgkin lymphomas were largely PRDM1-. Infiltrating T-cells in PRDM1- B-cell lymphomas expressed PRDM1. In conclusion, PRDM1 staining is a reliable and informative assay to define plasma cell commitment and differentiation in human normal and neoplastic B-cell lineages.

MeSH terms

  • Animals
  • Antigens, CD19 / analysis
  • Antigens, CD20
  • B-Lymphocytes / chemistry*
  • Biomarkers / analysis
  • CD3 Complex / analysis
  • Cell Differentiation
  • Cell Line
  • Cell Line, Transformed
  • Cell Lineage
  • DNA-Binding Proteins / analysis
  • Fluorescent Antibody Technique
  • Hodgkin Disease / metabolism*
  • Humans
  • Ki-1 Antigen / analysis
  • Lymphoma, B-Cell / chemistry
  • Membrane Glycoproteins / analysis
  • Mice
  • Neprilysin / analysis
  • PAX5 Transcription Factor
  • Plasma Cells / chemistry*
  • Positive Regulatory Domain I-Binding Factor 1
  • Proteoglycans / analysis
  • RNA, Messenger / analysis
  • Repressor Proteins / analysis*
  • Repressor Proteins / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Syndecan-1
  • Syndecans
  • T-Lymphocytes / chemistry
  • Transcription Factors / analysis*
  • Transcription Factors / genetics


  • Antigens, CD19
  • Antigens, CD20
  • Biomarkers
  • CD3 Complex
  • DNA-Binding Proteins
  • Ki-1 Antigen
  • Membrane Glycoproteins
  • PAX5 Transcription Factor
  • PAX5 protein, human
  • Pax5 protein, mouse
  • Prdm1 protein, mouse
  • Proteoglycans
  • RNA, Messenger
  • Repressor Proteins
  • SDC1 protein, human
  • Sdc1 protein, mouse
  • Syndecan-1
  • Syndecans
  • Transcription Factors
  • PRDM1 protein, human
  • Positive Regulatory Domain I-Binding Factor 1
  • Neprilysin