Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurones

J Physiol. 2005 Jun 15;565(Pt 3):897-910. doi: 10.1113/jphysiol.2004.081299. Epub 2005 Mar 17.


Increases in Ca(2+) concentration in the nucleus of neurones modulate gene transcription and may be involved in activity-dependent long-term plasticity, apoptosis, and neurotoxicity. Little is currently known about the regulation of Ca(2+) in the nuclei of neurones. Investigation of neuronal nuclei is hampered by the cellular heterogeneity of the brain where neurones comprise no more than 10% of the cells. The situation is further complicated by large differences in properties of different neurones. Here we report a method for isolating nuclei from identified central neurones. We employed this technique to study nuclei from rat cerebellar Purkinje and granule neurones. Patch-clamp recording from the nuclear membrane of Purkinje neurones revealed numerous large-conductance channels selective for monovalent cations. The nuclear membrane of Purkinje neurones also contained multiple InsP(3)- activated ion channels localized exclusively in the inner nuclear membrane with their receptor loci facing the nucleoplasm. In contrast, the nuclear membrane of granule neurones contained only a small number of mainly anion channels. Nuclear InsP(3) receptors (InsP(3)Rs) were activated by InsP(3) with EC(50) = 0.67 microm and a Hill coefficient of 2.5. Ca(2+) exhibited a biphasic effect on the receptors elevating its activity at low concentrations and inhibiting it at micromolar concentrations. InsP(3) in saturating concentrations did not prevent the inhibitory effect of Ca(2+), but strongly increased InsP(3)R activity at resting Ca(2+) concentrations. These data are the first evidence for the presence of intranuclear sources of Ca(2+) in neurones. Ca(2+) release from the nuclear envelope may amplify Ca(2+) transients penetrating the nucleus from the cytoplasm or generate Ca(2+) transients in the nucleus independently of the cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium Channels / physiology*
  • Cell Nucleus / physiology*
  • Cell Nucleus / ultrastructure
  • Inositol 1,4,5-Trisphosphate Receptors
  • Male
  • Membrane Potentials / physiology
  • Microscopy, Electron
  • Neurons / cytology
  • Neurons / physiology
  • Nuclear Envelope / physiology*
  • Nuclear Envelope / ultrastructure
  • Patch-Clamp Techniques
  • Purkinje Cells / cytology
  • Purkinje Cells / physiology*
  • Rats
  • Rats, Wistar
  • Receptors, Cytoplasmic and Nuclear / physiology*


  • Calcium Channels
  • Inositol 1,4,5-Trisphosphate Receptors
  • Receptors, Cytoplasmic and Nuclear
  • Calcium