Confirmation of the human cathepsin B gene (CTSB) assignment to chromosome 8

Hum Genet. 1992 Apr;89(1):10-2. doi: 10.1007/BF00207033.


Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cathepsin B / genetics*
  • Chromosomes, Human, Pair 8*
  • Cloning, Molecular
  • Fluorescence
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction


  • Cathepsin B