Characterization and immunomodulating activities of polysaccharide from Lentinus edodes

Int Immunopharmacol. 2005 May;5(5):811-20. doi: 10.1016/j.intimp.2004.11.011. Epub 2004 Dec 2.


The polysaccharide L-II was isolated and purified from the fruiting body of Lentinus edodes, which consisted of d-glucopyranose and had the molecular weight of 2.03 x 10(5) Da. We evaluated the effects of the polysaccharide L-II on the cellular immune response of Sarcoma 180-bearing mice. Mice were treated with three doses of the polysaccharide L-II (1, 5, and 10 mg/kg body weight) for 10 days. Tumor weight, relative spleen and thymus weight, delayed-type hypersensitivity (DTH) response, phagocytosis of macrophage, splenocytes proliferation were studied. Concentration of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) in mice serum were measured in control and polysaccharide groups. At the dose of 1, 5 and 10 mg/kg, a significant increase (p<0.05) in relative spleen and thymus weight, DTH, phagocytosis of macrophage was observed, as well as a significant decrease in tumor formation. The concentration of TNF-alpha, IFN-gamma in serum increased significantly in the polysaccharide groups compared with the model control group, but IL-2 not. Moreover, the polysaccharide L-II could increase NO production and catalase activity in macrophages. Results of these studies demonstrated the antitumor activity of the polysaccharide L-II on mice-transplanted sarcoma 180 was mediated by immunomodulation in inducing T-cells and macrophage-dependent immune system responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catalase / metabolism
  • Cytokines / biosynthesis
  • Female
  • Hypersensitivity, Delayed
  • Immunologic Factors / chemistry
  • Immunologic Factors / isolation & purification*
  • Immunologic Factors / pharmacology*
  • In Vitro Techniques
  • Lymphocyte Activation / drug effects
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / immunology
  • Macrophages, Peritoneal / metabolism
  • Mice
  • Molecular Weight
  • Nitric Oxide / biosynthesis
  • Phagocytosis / drug effects
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology*
  • Sarcoma 180 / drug therapy
  • Sarcoma 180 / immunology
  • Sarcoma 180 / metabolism
  • Sarcoma 180 / pathology
  • Shiitake Mushrooms / chemistry*


  • Cytokines
  • Immunologic Factors
  • Polysaccharides
  • Nitric Oxide
  • Catalase