Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein

Virology. 2005 Apr 10;334(2):160-5. doi: 10.1016/j.virol.2005.01.042.

Abstract

A secreted, glycosylated polypeptide containing amino acids 14 to 762 of the SARS coronavirus (SARS-CoV) spike protein and a polyhistidine tag was expressed in recombinant baculovirus-infected insect cells. Mice received the affinity-purified protein with either a saponin (QS21) or a Ribi (MPL + TDM) adjuvant subcutaneously and were challenged intranasally with SARS-CoV. Both regimens induced binding and neutralizing antibodies and protection against SARS-CoV intranasal infection. However, the best results were obtained with QS21 and protein, which provided the highest antibody as well as complete protection of the upper and lower respiratory tract.

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Baculoviridae
  • Cells, Cultured
  • HeLa Cells
  • Humans
  • Immunization
  • Membrane Glycoproteins / administration & dosage
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology*
  • Mice
  • Neutralization Tests
  • Peptides / administration & dosage
  • Peptides / chemistry
  • Peptides / genetics
  • Peptides / immunology*
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • SARS Virus / immunology*
  • SARS Virus / pathogenicity
  • Severe Acute Respiratory Syndrome / immunology
  • Severe Acute Respiratory Syndrome / prevention & control*
  • Solubility
  • Spike Glycoprotein, Coronavirus
  • Spodoptera
  • Viral Envelope Proteins / administration & dosage
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Membrane Glycoproteins
  • Peptides
  • Recombinant Proteins
  • Spike Glycoprotein, Coronavirus
  • Viral Envelope Proteins
  • Viral Vaccines
  • spike glycoprotein, SARS-CoV