Protection against cartilage proteoglycan synthesis inhibition by antiinterleukin 1 antibodies in experimental arthritis

J Rheumatol. 1992 Mar;19(3):348-56.


We have used neutralizing antibodies raised against murine recombinant interleukin 1 (IL-1) to demonstrate a role for IL-1 in the cartilage destruction and inflammation of antigen induced arthritis. Ex vivo production of IL-1 was demonstrated in tissue cultures of joint cross sections shortly after arthritis induction. Neutralizing antimurine IL-1 antibodies identified the activity to be about 80% IL-1 alpha 24 h after onset of arthritis. In animals receiving a single injection of anti-IL-1 antisera at Day -3, cartilage proteoglycan synthesis suppression during the first 2 days of arthritis was prevented. Normal proteoglycan synthesis was maintained until Day 4 when anti-IL-1 antisera was given at Days -2, 0, and 2 or arthritis. Dose response experiments showed that the reduction in inflammation was insufficient to account for the clearcut reduction in cartilage proteoglycan synthesis inhibition. Our results demonstrate that IL-1 plays a role in cartilage pathology in murine antigen induced arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / physiology*
  • Antibodies / therapeutic use
  • Arthritis / immunology
  • Arthritis / metabolism*
  • Arthritis / therapy
  • Cartilage, Articular / metabolism*
  • Immunotherapy
  • Interleukin-1 / biosynthesis
  • Interleukin-1 / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Neutralization Tests
  • Proteoglycans / antagonists & inhibitors*
  • Proteoglycans / biosynthesis
  • Serum Albumin, Bovine / immunology
  • Synovial Membrane / metabolism


  • Antibodies
  • Interleukin-1
  • Proteoglycans
  • Serum Albumin, Bovine