Potential ability of hot water adzuki (Vigna angularis) extracts to inhibit the adhesion, invasion, and metastasis of murine B16 melanoma cells

Biosci Biotechnol Biochem. 2005 Mar;69(3):448-54. doi: 10.1271/bbb.69.448.


The 40% ethanol eluent of the fraction of hot-water extract from adzuki beans (EtEx.40) adsorbed onto DIAION HP-20 resin has many biological activities, for example, antioxidant, antitumorigenesis, and intestinal alpha-glucosidase suppressing activities. This study examined the inhibitory effect of EtEx.40 on experimental lung metastasis and the invasion of B16-BL6 melanoma cells. EtEx.40 was found significantly to reduce the number of tumor colonies. It also inhibited the adhesion and migration of B16-BL6 melanoma cells into extracellular matrix components and their invasion into reconstituted basement membrane (matrigel) without affecting cell proliferation in vitro. These in vivo data suggest that EtEx.40 possesses a strong antimetastatic ability, which might be a lead compound in functional food development.

MeSH terms

  • Animals
  • Cell Adhesion / drug effects*
  • Cell Division / drug effects
  • Fabaceae / chemistry*
  • Hot Temperature
  • Melanoma, Experimental / pathology*
  • Mice
  • Neoplasm Invasiveness*
  • Plant Extracts / pharmacology*
  • Water / chemistry*


  • Plant Extracts
  • Water