Kinetic expression of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) during embryonic stem cell differentiation

J Cell Biochem. 2005 Jun 1;95(3):559-70. doi: 10.1002/jcb.20436.

Abstract

Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is widely used as a marker during vasculogenesis and angiogenesis from embryonic stem (ES) cells. However, the expression of PECAM-1 isoforms in ES cells has not been determined. The present study was designed to determine the role of PECAM-1 isoforms during in vitro endothelial differentiation of ES cells. It was found that undifferentiated ES cells expressed high level of PECAM-1, which primarily located at cell-cell junction, but the expression of PECAM-1 was sharply down-regulated during early ES cell differentiation. In addition, undifferentiated ES cells were found the expressed all eight known alternatively spliced PECAM-1 isoforms, among them the expression of PECAM-1 isoforms lacking exon 15 or 14&15 was predominant. Quantitative analysis revealed a significant increase in the expression of PECAM-1 isoform lacking exon 12&14&15 as vascular development of ES cells. These results indicate a constitutive expression of PECAM-1 in undifferentiated murine ES cells and suggest a developmental role of PECAM-1 isoform changes during vasculogenesis and angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology*
  • Cell Line
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism*
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / physiology*
  • Mice
  • Neovascularization, Pathologic / genetics
  • Neovascularization, Pathologic / metabolism*
  • Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis*
  • Platelet Endothelial Cell Adhesion Molecule-1 / genetics
  • Protein Isoforms / biosynthesis
  • Protein Isoforms / genetics
  • Stem Cells / cytology
  • Stem Cells / metabolism*

Substances

  • Platelet Endothelial Cell Adhesion Molecule-1
  • Protein Isoforms