DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus

Int J Med Microbiol. 2005 Mar;294(8):525-8. doi: 10.1016/j.ijmm.2004.11.002.


Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • Humans
  • Methicillin / pharmacology
  • Methicillin Resistance / genetics*
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / microbiology
  • Staphylococcal Protein A / genetics*
  • Staphylococcus aureus / classification
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification
  • Tandem Repeat Sequences / genetics*


  • Adhesins, Bacterial
  • DNA, Bacterial
  • Staphylococcal Protein A
  • clfB protein, Staphylococcus aureus
  • Methicillin