ATP-dependent Recognition of Eukaryotic Origins of DNA Replication by a Multiprotein Complex

Nature. 1992 May 14;357(6374):128-34. doi: 10.1038/357128a0.

Abstract

A multiprotein complex that specifically recognizes cellular origins of DNA replication has been identified and purified from the yeast Saccharomyces cerevisiae. We observe a strong correlation between origin function and origin recognition by this activity. Interestingly, specific DNA binding by the origin recognition complex is dependent upon the addition of ATP. We propose that the origin recognition complex acts as the initiator protein for S. cerevisiae origins of DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Base Sequence
  • Cell Nucleus / metabolism
  • Centrifugation, Density Gradient
  • Chromatography, Affinity
  • DNA Replication*
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / radiation effects
  • Electrophoresis, Polyacrylamide Gel
  • Macromolecular Substances
  • Molecular Sequence Data
  • Multiprotein Complexes
  • Oligodeoxyribonucleotides
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Ultraviolet Rays

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • Macromolecular Substances
  • Multiprotein Complexes
  • Oligodeoxyribonucleotides
  • Adenosine Triphosphate