Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron

FEBS Lett. 2005 Mar 28;579(9):1923-9. doi: 10.1016/j.febslet.2005.02.035.

Abstract

A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotinylation
  • Caco-2 Cells
  • Cation Transport Proteins / analysis
  • Cation Transport Proteins / genetics
  • Cation Transport Proteins / metabolism*
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Endosomes / chemistry
  • Endosomes / metabolism
  • Humans
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism*
  • Intestinal Mucosa / physiology
  • Iron / metabolism
  • Iron / pharmacology*
  • Iron-Binding Proteins / analysis
  • Iron-Binding Proteins / genetics
  • Iron-Binding Proteins / metabolism*
  • Lysosome-Associated Membrane Glycoproteins
  • Lysosomes / chemistry
  • Lysosomes / metabolism
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / metabolism

Substances

  • Cation Transport Proteins
  • Iron-Binding Proteins
  • LAMP1 protein, human
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Glycoproteins
  • solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2
  • Iron