Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae

Yeast. 1992 Jan;8(1):57-60. doi: 10.1002/yea.320080106.

Abstract

A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.

MeSH terms

  • Cell Division / drug effects
  • Cloning, Molecular
  • Endothelium, Vascular / drug effects
  • Humans
  • Pulmonary Artery / drug effects
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / pharmacology
  • Saccharomyces cerevisiae / genetics
  • Thymidine Phosphorylase / biosynthesis*
  • Thymidine Phosphorylase / pharmacology

Substances

  • Recombinant Proteins
  • Thymidine Phosphorylase