Hemagglutination and graft-versus-host disease in the severe combined immunodeficiency mouse lymphoproliferative disease model

Am J Pathol. 1992 May;140(5):1187-94.


In the course of evaluating the severe combined immunodeficiency mouse-human peripheral blood lymphocyte (SCID-PBL) model of lymphoproliferative disease, we noted hemagglutination occurring in peripheral blood smears of mice with serum human immunoglobulin levels greater than 1.0 mg/ml. The hemagglutinating process was mediated by human anti-mouse red cell antibodies of the IgM class, peaked at five to seven weeks post-transfer of 5 to 7 x 10(7) human PBL and was generally self limiting. However, death resulted in some mice when serum immunoglobulin levels were greater than 3.0 mg/ml. The most severely affected mice had hemagglutination induced congestion of liver, lungs and spleen. Several mice also had lesions consistent with graft-versus-host disease (GVHD) including focal hepatic necrosis and destruction of mouse splenic hematopoietic elements. The lesions associated with hemagglutination and GVHD in SCID-PBL mice are distinct from those associated with EBV-induced lymphoproliferation. Recognition of these pathologic processes are required for a thorough understanding of the SCID-PBL model.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Erythrocytes / metabolism
  • Flow Cytometry
  • Graft vs Host Disease / etiology*
  • Graft vs Host Disease / pathology
  • Hemagglutination*
  • Immunoglobulins / blood
  • Lymphoproliferative Disorders / blood*
  • Lymphoproliferative Disorders / complications
  • Lymphoproliferative Disorders / pathology
  • Mice
  • Mice, SCID
  • Severe Combined Immunodeficiency / blood*
  • Severe Combined Immunodeficiency / complications
  • Severe Combined Immunodeficiency / pathology


  • Immunoglobulins