Use of housekeeping gene sequencing for species identification of viridans streptococci

Diagn Microbiol Infect Dis. 2005 Apr;51(4):297-301. doi: 10.1016/j.diagmicrobio.2004.12.001.

Abstract

Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis-sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Typing Techniques*
  • DNA, Bacterial / analysis
  • Glucokinase / genetics*
  • Glucosephosphate Dehydrogenase / genetics*
  • Humans
  • Sequence Analysis, DNA*
  • Species Specificity
  • Viridans Streptococci / classification*
  • Viridans Streptococci / genetics

Substances

  • DNA, Bacterial
  • Glucosephosphate Dehydrogenase
  • Glucokinase