Techniques: high-throughput measurement of intracellular Ca(2+) -- back to basics

Trends Pharmacol Sci. 2005 Apr;26(4):218-23. doi: 10.1016/


High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, including the regulation and role of intracellular Ca(2+) in a plethora of biological systems. Although an awareness of specific assay conditions is crucial for reliable and reproducible measurements of intracellular free Ca(2+) whichever system of study is used, the complex temporal nature of Ca(2+) signals has posed some unique limitations for its measurement in high-throughput mode. Progress in high-throughput design has overcome many of these limitations and will complement other technical approaches to understanding the underlying regulation and role of intracellular Ca(2+).

Publication types

  • Review

MeSH terms

  • Animals
  • Calcium / analysis*
  • Calcium Signaling*
  • Cells, Cultured
  • Computational Biology / methods*
  • Drug Design*
  • Fluorometry
  • GTP-Binding Proteins / metabolism
  • Humans


  • GTP-Binding Proteins
  • Calcium