Analysis of circadian rhythms in zebrafish

Methods Enzymol. 2005;393:186-204. doi: 10.1016/S0076-6879(05)93005-X.

Abstract

The zebrafish probably constitutes the best animal system to study the complexity of the circadian clock machinery and the influence that light has on it. The possibilities of producing transgenic fishes, to establish light-responsive cultured cells, and to directly explore light phototransduction on single clock cells are all remarkable features of this circadian system. This article describes some of the most useful methodologies to analyze the behavioral, cellular, and molecular aspects of the zebrafish circadian clock system.

MeSH terms

  • Animals
  • Behavior, Animal
  • Cell Culture Techniques / methods
  • Cell Line
  • Circadian Rhythm / physiology*
  • Circadian Rhythm / radiation effects
  • Genetic Techniques
  • Larva / growth & development
  • Luminescent Measurements / methods
  • Organ Culture Techniques / methods
  • Photic Stimulation
  • Signal Transduction / radiation effects
  • Transgenes
  • Zebrafish / genetics
  • Zebrafish / physiology*
  • Zebrafish Proteins / isolation & purification

Substances

  • Zebrafish Proteins