D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut

J Neurobiol. 2005 Aug;64(2):157-69. doi: 10.1002/neu.20131.


By database searches of the Drosophila genome project we have identified D-hil as the fly member of a novel family of W180-domain containing proteins. Immunocytochemistry demonstrated that D-hil is localized to the neuropil of the embryonic CNS, to the cellular cortex of dividing neuroblasts from larval brains, and that it is up-regulated in the cleavage furrow of S2 cells. We show that D-hil distribution overlaps extensively with that of the septin family member Pnut. Cross-immunoprecipitation experiments further indicated that the two proteins may be members of the same protein complex. Analysis of a severe hypomorphic P-element mutation in the D-hil locus suggested that D-hil is a nonessential protein. However, by creating double mutant flies we show that the D-hil locus acts as a modulator of Pnut function by increasing the level of polyploidy of neuroblasts in Pnut(KG00478)/Pnut(KG00478) larval brains. Based on these results we propose that D-hil may function as a regulator of septin function during cytokinesis in the developing nervous system.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Blotting, Western / methods
  • Brain / embryology
  • Brain / metabolism*
  • Cell Line
  • Cytokinesis / physiology*
  • Drosophila
  • Drosophila Proteins / metabolism
  • Drosophila Proteins / physiology*
  • Gene Expression Regulation, Developmental / physiology
  • Genomic Imprinting
  • Green Fluorescent Proteins / metabolism
  • Immunohistochemistry / methods
  • Immunoprecipitation / methods
  • Larva
  • Microfilament Proteins / metabolism*
  • Molecular Sequence Data
  • Mutagenesis / physiology
  • Nerve Tissue Proteins / physiology*
  • Phylogeny
  • Protein Biosynthesis
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Time Factors
  • Transfection / methods


  • Drosophila Proteins
  • Microfilament Proteins
  • Nerve Tissue Proteins
  • hillarin
  • pnut protein, Drosophila
  • Green Fluorescent Proteins