c-src activating mutation analysis in Chinese patients with colorectal cancer

World J Gastroenterol. 2005 Apr 21;11(15):2351-3. doi: 10.3748/wjg.v11.i15.2351.

Abstract

Aim: To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland.

Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases.

Results: Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found.

Conclusion: c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.

MeSH terms

  • Adult
  • Aged
  • Asian Continental Ancestry Group / genetics*
  • Colorectal Neoplasms / ethnology
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / pathology
  • Genes, src / genetics*
  • Humans
  • Liver Neoplasms / ethnology
  • Liver Neoplasms / genetics
  • Liver Neoplasms / secondary
  • Middle Aged
  • Point Mutation
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single-Stranded Conformational