Isolation and analysis of candidate myeloid tumor suppressor genes from a commonly deleted segment of 7q22

Genomics. 2005 May;85(5):600-7. doi: 10.1016/j.ygeno.2005.01.013.

Abstract

Monosomy 7 and deletions of 7q are recurring leukemia-associated cytogenetic abnormalities that correlate with adverse outcomes in children and adults. We describe a 2.52-Mb genomic DNA contig that spans a commonly deleted segment of chromosome band 7q22 identified in myeloid malignancies. This interval currently includes 14 genes, 19 predicted genes, and 5 predicted pseudogenes. We have extensively characterized the FBXL13, NAPE-PLD, and SVH genes as candidate myeloid tumor suppressors. FBXL13 encodes a novel F-box protein, SVHis a member of a gene family that contains Armadillo-like repeats, and NAPE-PLD encodes a phospholipase D-type phosphodiesterase. Analysis of a panel of leukemia specimens with monosomy 7 did not reveal mutations in these or in the candidate genes LRRC17, PRO1598, and SRPK2. This fully sequenced and annotated contig provides a resource for candidate myeloid tumor suppressor gene discovery.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • Bone Marrow Neoplasms / genetics*
  • Chromosome Deletion*
  • Chromosomes, Human, Pair 7 / genetics*
  • Cloning, Molecular
  • Computational Biology
  • Contig Mapping
  • DNA, Complementary / genetics
  • Databases, Nucleic Acid
  • Genes, Tumor Suppressor*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Polymorphism, Single-Stranded Conformational
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Trans-Activators / genetics*

Substances

  • ARMC10 protein, human
  • DNA, Complementary
  • Neoplasm Proteins
  • Trans-Activators