Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica

Eukaryot Cell. 2005 Apr;4(4):827-31. doi: 10.1128/EC.4.4.827-831.2005.

Abstract

Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism
  • Animals
  • Biomarkers / metabolism
  • Entamoeba histolytica / metabolism*
  • GTP Phosphohydrolases / metabolism
  • Phagocytosis*
  • Proteomics*
  • Protozoan Proteins / metabolism
  • Protozoan Proteins / physiology*
  • rab7 GTP-Binding Proteins

Substances

  • Actins
  • Biomarkers
  • Protozoan Proteins
  • rab7 GTP-Binding Proteins
  • GTP Phosphohydrolases
  • Rab7 protein, Entamoeba histolytica