Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport

Nat Cell Biol. 2005 May;7(5):483-92. doi: 10.1038/ncb1246. Epub 2005 Apr 10.


Cortactin is an actin-binding protein that has recently been implicated in endocytosis. It binds directly to dynamin-2 (Dyn2), a large GTPase that mediates the formation of vesicles from the plasma membrane and the Golgi. Here we show that cortactin associates with the Golgi to regulate the actin- and Dyn2-dependent transport of cargo. Cortactin antibodies stain the Golgi apparatus, labelling peripheral buds and vesicles that are associated with the cisternae. Notably, in vitro or intact-cell experiments show that activation of Arf1 mediates the recruitment of actin, cortactin and Dyn2 to Golgi membranes. Furthermore, selective disruption of the cortactin-Dyn2 interaction significantly reduces the levels of Dyn2 at the Golgi and blocks the transit of nascent proteins from the trans-Golgi network, resulting in swollen and distended cisternae. These findings support the idea of an Arf1-activated recruitment of an actin, cortactin and Dyn2 complex that is essential for Golgi function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ADP-Ribosylation Factor 1 / genetics
  • ADP-Ribosylation Factor 1 / metabolism*
  • Actins / metabolism*
  • Animals
  • Cells, Cultured
  • Cortactin
  • Dynamin II / genetics
  • Dynamin II / metabolism*
  • Endoplasmic Reticulum / genetics
  • Endoplasmic Reticulum / metabolism
  • Endoplasmic Reticulum / ultrastructure
  • Fluorescent Antibody Technique, Indirect
  • Golgi Apparatus / genetics
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / ultrastructure
  • Macromolecular Substances / metabolism
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Microinjections
  • Microscopy, Immunoelectron
  • Protein Transport / physiology
  • Rats


  • Actins
  • Cortactin
  • Cttn protein, rat
  • Macromolecular Substances
  • Microfilament Proteins
  • ADP-Ribosylation Factor 1
  • Dynamin II