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, 12 (5), 408-16

Ribosomal Protein L30 Is a Component of the UGA-selenocysteine Recoding Machinery in Eukaryotes

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Ribosomal Protein L30 Is a Component of the UGA-selenocysteine Recoding Machinery in Eukaryotes

Laurent Chavatte et al. Nat Struct Mol Biol.

Abstract

The translational recoding of UGA as selenocysteine (Sec) is directed by a SECIS element in the 3' untranslated region (UTR) of eukaryotic selenoprotein mRNAs. The selenocysteine insertion sequence (SECIS) contains two essential tandem sheared G.A pairs that bind SECIS-binding protein 2 (SBP2), which recruits a selenocysteine-specific elongation factor and Sec-tRNA(Sec) to the ribosome. Here we show that ribosomal protein L30 is a component of the eukaryotic selenocysteine recoding machinery. L30 binds SECIS elements in vitro and in vivo, stimulates UGA recoding in transfected cells and competes with SBP2 for SECIS binding. Magnesium, known to induce a kink-turn in RNAs that contain two tandem G.A pairs, decreases the SBP2-SECIS complex in favor of the L30-SECIS interaction. We propose a model in which SBP2 and L30 carry out different functions in the UGA recoding mechanism, with the SECIS acting as a molecular switch upon protein binding.

Comment in

  • Knowing when not to stop.
    Berry MJ. Berry MJ. Nat Struct Mol Biol. 2005 May;12(5):389-90. doi: 10.1038/nsmb0505-389. Nat Struct Mol Biol. 2005. PMID: 15870727 No abstract available.

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