[Immunoglobulin isotype and IgG subclass profiles in American tegumentary leishmaniasis]

Rev Soc Bras Med Trop. Mar-Apr 2005;38(2):137-41. doi: 10.1590/s0037-86822005000200002. Epub 2005 Mar 30.
[Article in Portuguese]

Abstract

The present work investigated the serum antibody profiles in 37 patients with American tegumentary leishmaniasis, who were attended at Hospital de Clinicas - Universidade Federal de Uberlandia, MG, Brazil. The immunoglobulin class and IgG subclass profiles were analyzed by indirect ELISA using Leishmania (Leishmania) amazonensis soluble antigen. The antibody avidity was determined by 6 M urea treatment after incubation with immunoenzymatic conjugate. It was observed that 97% of the serum samples presented anti-Leishmania antibodies for IgE class, 94.6% IgG, 57.5% IgA and 21.5% IgM class. For IgG subclasses the profiles were in the following order of frequency: IgG1>IgG3>IgG2>IgG4. High avidity of anti-Leishmania IgE antibodies was found in 44.4% of the samples. On the other hand, moderate avidity of specific IgG and IgA was observed in 62.8% and 47.8% of samples, respectively. These results indicate a very complex antibody response profile against American tegumentary leishmaniasis.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aged
  • Animals
  • Antibodies, Protozoan / blood*
  • Antibody Affinity
  • Biomarkers / blood
  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / classification
  • Immunoglobulin G / immunology
  • Immunoglobulin Isotypes / blood
  • Immunoglobulin Isotypes / immunology*
  • Immunoglobulins / blood*
  • Immunoglobulins / immunology
  • Leishmania braziliensis / immunology*
  • Leishmaniasis, Cutaneous / blood
  • Leishmaniasis, Cutaneous / immunology*
  • Male
  • Middle Aged

Substances

  • Antibodies, Protozoan
  • Biomarkers
  • Immunoglobulin G
  • Immunoglobulin Isotypes
  • Immunoglobulins