Co-transfection of messenger RNA and siRNA as a method to study the efficiency of siRNA

Nucleosides Nucleotides Nucleic Acids. 2005;24(2):147-52. doi: 10.1081/NCN-51908.


The definition of an optimal siRNA results from the in vitro testing of several siRNA designed to specifically target a gene. Usually, such in vitro tests consist in the transfection of the several siRNA duplexes in a cell expressing stably the gene of interest. When a siRNA specific for a mRNA coding toxic proteins (certain transcription factors, transporters, toxins, cell cycle controlling proteins, etc.) must be tested, the generation of a target cell is difficult. Here we report a quick method to test the efficiency of a siRNA through its co-transfection with the targeted mRNA. This technique can be used as a fast method to test siRNA even when they target genes that cannot be stably expressed in the cells of interest.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Dendritic Cells
  • Electroporation
  • Gene Expression
  • Mice
  • RNA Interference*
  • RNA, Messenger / genetics*
  • RNA, Small Interfering / genetics*
  • Transfection


  • RNA, Messenger
  • RNA, Small Interfering