Synonymous codon usage and gene function are strongly related in Oryza sativa

Biosystems. 2005 May;80(2):123-31. doi: 10.1016/j.biosystems.2004.10.008. Epub 2004 Dec 8.

Abstract

The relationship between codon usage and gene function was investigated while considering a dataset of 2106 nuclear genes of Oryza sativa. The results of standard chi(2) test and F-statistic showed that for every 59 synonymous codons, a strongly significant association with gene functional categories existed in rice, indicating that codon usage was generally coordinated with gene function whether it was at the level of individual amino acids or at the level of nucleotides. However, it could not be directly said that the use of every codons differed significantly between any two functional categories. Notably, there existed large difference both in selection for biased codons or selection intensity among functional categories. Therefore, we identified at least two classes of genes: one group of genes, mainly belonging to the "METABOLISM" category, was tended to use G- and/or C-ending codons while the other was more biased to choose codons ending with A and/or U. The latter group contained genes of various functions, especially those genes classified into the "Nuclear Structure" category. These observations will be more important for molecular genetic engineering and genome functional annotation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping / methods*
  • Codon / genetics*
  • Genome, Plant
  • Oryza / genetics*
  • Oryza / metabolism*
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism*
  • Sequence Analysis, DNA / methods*
  • Structure-Activity Relationship

Substances

  • Codon
  • Plant Proteins