Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, a dietary legume

Biochem Biophys Res Commun. 2005 May 20;330(4):1268-74. doi: 10.1016/j.bbrc.2005.03.109.


Development of nontoxic and biologically safe antiangiogenic agent has been highlighted as a promising way to treat angiogenesis related diseases including cancer. Herein, we isolated 4-O-methylgallic acid (4-OMGA) from the seed of Canavalia gladiata, a dietary legume, on the basis of the growth inhibitory activity for bovine aortic endothelial cells (BAECs). The compound potently inhibits endothelial cell invasion and tube formation stimulated with basic fibroblast growth factor (bFGF) at low micromolar concentrations where it shows no cytotoxicity to the cells. In addition, 4-OMGA inhibits vascular endothelial cell growth factor (VEGF) production under hypoxic condition and the production of reactive oxygen species (ROS) in the endothelial cells stimulated with VEGF. These results demonstrate that 4-OMGA is a compound having potential for an antiangiogenic agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / isolation & purification
  • Angiogenesis Inhibitors / pharmacology*
  • Animals
  • Aorta / cytology
  • Aorta / drug effects
  • Canavalia / chemistry*
  • Cattle
  • Cell Hypoxia
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Fibroblast Growth Factor 2 / pharmacology
  • Gallic Acid / analogs & derivatives*
  • Gallic Acid / isolation & purification
  • Gallic Acid / pharmacology*
  • Humans
  • Plant Extracts / chemistry
  • Reactive Oxygen Species / metabolism
  • Seeds / chemistry
  • Vascular Endothelial Growth Factor A / biosynthesis
  • Vascular Endothelial Growth Factor A / pharmacology


  • Angiogenesis Inhibitors
  • Plant Extracts
  • Reactive Oxygen Species
  • Vascular Endothelial Growth Factor A
  • 3,5-dihydroxy-4-methoxybenzoic acid
  • Fibroblast Growth Factor 2
  • Gallic Acid