Primary structure and functional expression of the Na/Ca,K-exchanger from bovine rod photoreceptors

EMBO J. 1992 May;11(5):1689-95.

Abstract

Complementary DNA encoding the Na/Ca,K-exchanger was isolated from bovine retina cDNA libraries. The complete full-length cDNA is approximately 4 kb long and contains an open reading frame of 3597 bp. The deduced amino acid sequence corresponds to a protein of 1199 amino acids with a calculated molecular weight of approximately 130 kDa. Hydrophobicity analysis revealed the presence of two alternating sets of hydrophobic and hydrophilic domains. There also exists a hydrophobic region at the N-terminus which may be part of a cleavable signal peptide. The protein shares limited sequence homology with the Na/Ca-exchanger from cardiac sarcolemma. Northern blot analysis indicates that the approximately 6 kb transcript is highly specific for retinal tissue. Insect cells infected with recombinant baculovirus bearing the full-length cDNA express a functional Na/Ca,K-exchanger with an apparent relative molecular weight of approximately 210 kDa, as determined by Western blotting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Cattle
  • Cloning, Molecular
  • DNA / genetics
  • Molecular Sequence Data
  • Myocardium / metabolism
  • Open Reading Frames
  • Photoreceptor Cells / metabolism*
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Rod Cell Outer Segment / metabolism*
  • Sequence Homology, Nucleic Acid
  • Sodium-Calcium Exchanger*

Substances

  • Carrier Proteins
  • RNA, Messenger
  • Sodium-Calcium Exchanger
  • potassium-dependent sodium-calcium exchanger
  • DNA

Associated data

  • GENBANK/S35174
  • GENBANK/S35177
  • GENBANK/X67719
  • GENBANK/X67720
  • GENBANK/X67721
  • GENBANK/X67722
  • GENBANK/X67723
  • GENBANK/X67724
  • GENBANK/X67725
  • GENBANK/X67726