Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells

J Virol. 2005 May;79(9):5857-62. doi: 10.1128/JVI.79.9.5857-5862.2005.


Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Cells, Cultured
  • Distemper Virus, Canine / physiology*
  • Ferrets
  • Glycoproteins*
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / physiology*
  • Immunoglobulins*
  • Leukocytes, Mononuclear / virology
  • Membrane Fusion
  • Models, Molecular
  • Molecular Sequence Data
  • Receptors, Cell Surface
  • Receptors, Virus*
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • Virus Replication


  • Antigens, CD
  • Glycoproteins
  • Hemagglutinins, Viral
  • Immunoglobulins
  • Receptors, Cell Surface
  • Receptors, Virus
  • SLAMF1 protein, human
  • Signaling Lymphocytic Activation Molecule Family Member 1