Abstract
Terminally sialylated oligosaccharides were synthesised employing recombinant trans-sialidase from Trypanosoma cruzi. Regio- and stereoselectively Sia-alpha(2-3)-Gal-betaR derivatives could be obtained in respectable yields, using combined chemical and enzymatic methodologies. An array of different disaccharide precursors such as Gal-beta(1-3)-GalNAc-alphaSer/Thr, lactosides and lactosamide derivatives were sialylated and successfully purified by facile isolation procedures. Depending on the acceptor structure isolated, yields for trans-sialylation products were between 20 and 60%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carbohydrate Conformation
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Cattle
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Glycoproteins / genetics
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Glycoproteins / metabolism*
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Glycosylation
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Male
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Neuraminidase / genetics
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Neuraminidase / metabolism*
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Oligosaccharides / chemical synthesis
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Oligosaccharides / chemistry*
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Oligosaccharides / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sialic Acids / chemistry*
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Substrate Specificity
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Testis / enzymology
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Trypanosoma cruzi / enzymology*
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beta-Galactosidase / metabolism
Substances
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Glycoproteins
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Oligosaccharides
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Recombinant Proteins
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Sialic Acids
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trans-sialidase
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Neuraminidase
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beta-Galactosidase