[Biocompatibility study on tendon mixed extraction of bovine collagen for periodontal tissue engineering]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Mar 15;19(3):234-7.
[Article in Chinese]

Abstract

Objective: To study the biocompatibility of tendon mixed extraction of bovine collagen (tMEBC) and to explore the feasibility of using the three-dimensional framework as periodontal tissue engineering scaffold.

Methods: After being prepared, the tMEBC were cultured with the P4-P6 of human periodontal ligament fibroblasts (HPDLFs) in vitro. Three-dimensional framework was prepared from bovine tendon. The P4-P6 of HPDLFs (with an initial density of 5 x 10(6) cells/ml) were cultured in vitro. Cell attachment and proliferation were measured by cell counting 1 day, 3, 5, and 10 days after cell seeding. Histological examination was performed with light microscope and scanning electron microscope 5 and 10 days after cell seeding.

Results: Porous structure, which supported the proliferation and attachment of HPDLFs, was found in tMEBC. The density of cell increased from 0.556 x 10(4) cells/ml 24 hours after cell seeding to 3.944 X 10(4) cells/mI 10 days after seeding. Light and scanning electron microscope examination indicated that HPDLFs were attached and extended on the three-dimensional scaffolds and were well embedded in the newly formed tissue matrix.

Conclusion: tMEBC has good biocompatibility with the HPDLFs, and can be used as scaffold for cell transplantation in periodontal tissue engineering.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Cattle
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Collagen / chemistry*
  • Feasibility Studies
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / ultrastructure
  • Humans
  • Microscopy, Electron, Scanning
  • Periodontal Ligament / cytology*
  • Tendons / chemistry*
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry*

Substances

  • Biocompatible Materials
  • Collagen