Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma

Proteomics. 2005 May;5(7):1980-6. doi: 10.1002/pmic.200401084.


A murine monoclonal antibody (mAb), CLD3 (IgG(1),kappa), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix-assisted laser desorption/ionization-time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / metabolism*
  • Antibodies, Neoplasm / metabolism
  • Antigens, Nuclear / immunology
  • Antigens, Nuclear / metabolism*
  • Binding Sites, Antibody
  • Carcinoma, Hepatocellular / chemistry
  • Carcinoma, Hepatocellular / immunology*
  • DNA-Binding Proteins / immunology
  • DNA-Binding Proteins / metabolism*
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Ku Autoantigen
  • Liver Neoplasms / immunology*
  • Liver Neoplasms / metabolism
  • Molecular Sequence Data
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Antigens, Nuclear
  • DNA-Binding Proteins
  • Xrcc6 protein, human
  • Ku Autoantigen