Nucleotide Sequences and Biologic Properties of Toxic Shock Syndrome Toxin 1 From Ovine- And Bovine-Associated Staphylococcus Aureus

J Infect Dis. 1992 Jun;165(6):1056-63. doi: 10.1093/infdis/165.6.1056.


Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pI) of 8.5, whereas TSST-bovine has the same pI (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Toxins*
  • Base Sequence
  • Blotting, Western
  • Cattle
  • DNA, Bacterial / chemistry
  • Enterotoxins / chemistry
  • Enterotoxins / genetics*
  • Female
  • Humans
  • Lymphocyte Activation
  • Mastitis / microbiology
  • Mastitis / veterinary*
  • Mastitis, Bovine / microbiology*
  • Molecular Sequence Data
  • Rabbits
  • Sheep
  • Sheep Diseases / microbiology*
  • Shock, Septic / etiology
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary
  • Staphylococcus aureus*
  • Superantigens*


  • Bacterial Toxins
  • DNA, Bacterial
  • Enterotoxins
  • Superantigens
  • enterotoxin F, Staphylococcal