Serologic Diagnosis of Lyme Disease Using Recombinant Outer Surface Proteins A and B and Flagellin

J Infect Dis. 1992 Jun;165(6):1127-32. doi: 10.1093/infdis/165.6.1127.

Abstract

Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antibodies, Monoclonal / immunology
  • Antigens, Bacterial / immunology
  • Bacterial Outer Membrane Proteins / immunology*
  • Borrelia burgdorferi Group / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Flagellin / immunology*
  • Humans
  • Immunoblotting
  • Lyme Disease / diagnosis*
  • Recombinant Fusion Proteins / immunology
  • Recombinant Proteins / immunology

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Flagellin