Expression of Matrix metalloproteinase-10 in Human Bladder Transitional Cell Carcinoma

Urology. 2005 Apr;65(4):815-20. doi: 10.1016/j.urology.2004.11.016.

Abstract

Objectives: To analyze matrix metalloproteinase-10 (MMP-10) expression in transitional cell carcinoma (TCC) of the bladder, evaluate the correlations between MMP-10 protein expression and clinicopathologic parameters, and address the viability of MMP-10 as a therapeutic target for TCC. MMP-mediated degradation of the extracellular matrix is an important factor in the pathogenesis of tumorigenesis and metastasis.

Methods: Using immunohistochemistry, the expression of MMP-10 was assessed using both tissue microarrays and whole sections of archival tissue specimens representative of all grades and stages of human bladder TCC (n = 60). MMP-10 expression was also assessed in histologically normal human bladder tissue (n = 10). The immunostaining results for MMP-10 expression were examined for correlations with tumor grade and stage.

Results: Unlike most MMPs, MMP-10 was localized primarily in the tumor mass as opposed to the tumor stroma and was detectable in all grades and stages of TCC. Significantly greater levels of MMP-10 protein were observed in superficial (pTa, pT1; n = 38) tumors than in normal bladder tissue (P = 0.01). In contrast to the proposed role of MMPs in tumor invasion, no significant difference was observed between muscle-invasive tumors (pT2 or worse; n = 22) and histologically normal bladder tissue (P = 0.50). MMP-10 expression showed no significant correlation with tumor grade.

Conclusions: The data from our study showed that, unlike most MMPs, MMP-10 was not associated with tumor aggression or invasion. Our results suggest that MMP-10 protein levels are significantly greater in the earlier stages of TCC development.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carcinoma, Transitional Cell / chemistry
  • Carcinoma, Transitional Cell / metabolism*
  • Humans
  • Matrix Metalloproteinase 10
  • Metalloendopeptidases / analysis
  • Metalloendopeptidases / biosynthesis*
  • Urinary Bladder Neoplasms / chemistry
  • Urinary Bladder Neoplasms / metabolism*

Substances

  • Metalloendopeptidases
  • Matrix Metalloproteinase 10