RNA duplexes with biphenyl substituents as base replacements are less stable than DNA duplexes

Chem Commun (Camb). 2005 Apr 21:(15):2023-5. doi: 10.1039/b419278h. Epub 2005 Feb 28.

Abstract

Introduction of a hydrophobic biphenyl-C-nucleotide pair into a 11-mer RNA duplex is associated with a net penalty in the free energy of duplex formation of 2.0 kcal mol(-1) or 10 degrees C in Tm, relative to DNA. These differential stabilities are of relevance with respect to the transcriptional and translational aspects of hydrophobic base-pairs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Pairing
  • Biphenyl Compounds / chemistry*
  • Circular Dichroism
  • DNA / chemistry*
  • RNA / chemistry*
  • Transition Temperature / radiation effects

Substances

  • Biphenyl Compounds
  • biphenyl
  • RNA
  • DNA