Enhanced radical scavenging activity of genetically modified Arabidopsis seeds

Biotechnol Lett. 2005 Mar;27(5):297-303. doi: 10.1007/s10529-005-0683-7.


The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1 cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics
  • Biotechnology
  • Cell Differentiation
  • Chromatography, High Pressure Liquid
  • DNA, Complementary / metabolism
  • Dose-Response Relationship, Drug
  • Flavonoids / metabolism
  • Free Radicals
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Genetic Engineering / methods*
  • Models, Chemical
  • Mutation
  • Pancreatitis-Associated Proteins
  • Plants, Genetically Modified*
  • Proanthocyanidins / metabolism
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins c-myb / chemistry
  • Recombinant Fusion Proteins / chemistry
  • Seeds
  • Spectrometry, Mass, Electrospray Ionization
  • Time Factors


  • Arabidopsis Proteins
  • DNA, Complementary
  • Flavonoids
  • Free Radicals
  • Pancreatitis-Associated Proteins
  • Proanthocyanidins
  • Proto-Oncogene Proteins c-myb
  • REG3A protein, human
  • Recombinant Fusion Proteins
  • proanthocyanidin