Brain arachidonic acid incorporation is decreased in heart fatty acid binding protein gene-ablated mice

Biochemistry. 2005 Apr 26;44(16):6350-60. doi: 10.1021/bi047292r.


Heart fatty acid binding protein (H-FABP) is expressed in neurons, but its role in brain fatty acid incorporation and metabolism is poorly defined. We examined the effect of H-FABP gene ablation on brain incorporation of arachidonic ([1-(14)C]20:4n-6) or palmitic ([1-(14)C]16:0) acid in vivo. Analysis of brain mRNA confirmed gene ablation and demonstrated no compensatory changes in the levels of other FABP mRNA in the gene-ablated mice. In brains from H-FABP gene-ablated mice, the incorporation coefficient for [1-(14)C]20:4n-6 was reduced 24%, while that for [1-(14)C]16:0 was unaffected. Within the organic and aqueous fractions, significantly more [1-(14)C]20:4n-6 was distributed into the aqueous fraction, suggesting a disruption in the metabolic targeting of 20:4n-6 in these mice. There was less incorporation of [1-(14)C]20:4n-6 into total phospholipids and a marked reduction (51%) in the level of incorporation into the choline glycerophospholipids (ChoGpl). Because FABP can influence steady-state lipid mass, brain individual lipid masses were measured. The brain total phospholipid mass was reduced 17% by gene ablation, ascribed to a 27% and 32% reduction in the masses of ChoGpl and sphingomyelin, respectively. Plasmalogen subclass masses were also reduced, suggesting that H-FABP may augment brain plasmalogen synthesis. In gene-ablated mice, the phosphatidylinositol 20:4n-6 level was reduced 25%, while the proportion of total n-6 fatty acids was reduced in the major phospholipid classes. Thus, these results demonstrate for the first time that H-FABP expression influences brain 20:4n-6 uptake and trafficking as well as steady-state brain lipid levels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism*
  • Base Sequence
  • Brain / metabolism*
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • DNA / genetics
  • Fatty Acid-Binding Proteins
  • Fatty Acids / metabolism
  • Gene Expression
  • Kinetics
  • Male
  • Mice
  • Mice, Knockout
  • Phospholipids / chemistry
  • Phospholipids / metabolism
  • Plasmalogens / chemistry
  • Plasmalogens / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism


  • Carrier Proteins
  • Fatty Acid-Binding Proteins
  • Fatty Acids
  • Phospholipids
  • Plasmalogens
  • RNA, Messenger
  • Arachidonic Acid
  • DNA