The double disector: unbiased stereological estimation of the number of particles inside other particles

J Microsc. 1992 Mar;165(Pt 3):417-26. doi: 10.1111/j.1365-2818.1992.tb01496.x.

Abstract

A double-disector method for obtaining estimates of the number of particles inside other particles, illustrated by an estimation of the average cell number in a glomerulus, has been developed. The method is suitable for paraffin-embedded tissue because it does not require knowledge about the section thickness. Most importantly, the estimate is absolutely unaffected by tissue shrinkage. The average number of cells in a human glomerulus is 2850 whereas in rats it is 580.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Count
  • Humans
  • Kidney Glomerulus / cytology*
  • Male
  • Microscopy / methods*
  • Rats
  • Rats, Inbred Strains