Crystallization of the exo(1,3)-beta-glucanase from Candida albicans

J Mol Biol. 1992 May 5;225(1):217-8. doi: 10.1016/0022-2836(92)91037-p.

Abstract

An exoglucanase, with specificity for beta (1,3) linkages, from the cell wall of Candida albicans has been crystallized by the hanging drop method in the presence of polyethylene glycol 8000. The crystals, which diffract to better than 1.9 A resolution, belong to the orthorhombic space group P212121 with cell constants a = 60.2 A, b = 65.2 A, c = 96.5 A and with one molecule in the asymmetric unit.

MeSH terms

  • Candida albicans / enzymology*
  • Crystallization
  • Glucan 1,3-beta-Glucosidase
  • X-Ray Diffraction
  • beta-Glucosidase / chemistry*
  • beta-Glucosidase / isolation & purification
  • beta-Glucosidase / metabolism

Substances

  • beta-Glucosidase
  • Glucan 1,3-beta-Glucosidase