Immune Responses in Balb/c Mice Induced by a Candidate SARS-CoV Inactivated Vaccine Prepared From F69 Strain

Vaccine. 2005 May 2;23(24):3196-201. doi: 10.1016/j.vaccine.2004.11.073.

Abstract

The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Antibodies, Viral / biosynthesis*
  • Antibody Specificity
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Immunization
  • Immunoglobulin G / analysis
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / analysis
  • Immunoglobulin M / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neutralization Tests
  • SARS Virus / immunology*
  • Severe Acute Respiratory Syndrome / immunology*
  • Severe Acute Respiratory Syndrome / virology
  • Vaccines, Inactivated
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • Vaccines, Inactivated
  • Viral Vaccines

Associated data

  • GENBANK/AY313906
  • GENBANK/AY394989