Detection and discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA amplification

Microbiol Immunol. 1992;36(1):21-7. doi: 10.1111/j.1348-0421.1992.tb01638.x.


By using the primers designed on the bases of the sequences of the 16S rRNA genes of Mycoplasma pneumoniae and Mycoplasma genitalium, respectively, specific and sensitive in vitro DNA amplification assay system for the detection and discrimination of these two mycoplasmas was established. The detection limit of the assay was 100 cells for M. pneumoniae and 1,000 cells for M. genitalium. Neither other human mycoplasmas nor oral bacteria existing in human saliva showed any cross-reactions with these primers.

MeSH terms

  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Humans
  • Molecular Sequence Data
  • Mycoplasma / genetics
  • Mycoplasma / isolation & purification*
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification*
  • Polymerase Chain Reaction
  • Species Specificity


  • DNA, Bacterial